The eight-year-old girl died in hospital earlier today, West Yorkshire Police said.
A 10-year-old girl remains in hospital in a critical condition.
Police were called by West Yorkshire Fire and Rescue Service (WYFRS) to the incident on Leeds Road, Huddersfield, just before 2.25am today.
A woman and two children were rescued from the property and taken to hospital with life-threatening injuries.
The 31-year-old woman died from her injuries earlier this morning.
A spokesperson for West Yorkshire Police said: “Investigations into the fire are in their early stages.
“We are currently treating it as suspicious.
“However, we are not currently looking for anyone else in connection with the incident.”
Detective Chief Inspector James Entwistle, from the homicide and major enquiry team, said: “Our thoughts go out to everyone affected by this tragic incident.
“Specially-trained officers are supporting the family.
“While we are not currently looking for anyone else in connection with the incident, we are still keen to speak to anyone who was in the area this morning, who may have seen something, to come forward with information.
“A scene remains in place while enquiries are carried out.”
Anyone with information should contact the homicide and major enquiry team by calling 101.
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