The A47 was closed between the A1122 and the A1065 at Swaffham.
The collision, between a car and a van, happened at 10.50am near the roundabout heading eastbound towards Swaffham.
Three fire crews and two ambulances attended and five patients were assessed at the scene.
Two were taken to the Queen Elizabeth Hospital for further treatment and the other three patients, who suffered minor injuries, were discharged.
National Highways East confirmed that all lanes will soon be reopened before 5pm today.
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